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GATE Biotechnology 2013 Previous Questions and Answers

1 Under alkaline conditions, DNA is more stable than RNA because
GATE  Biotechnology
(A) RNA forms secondary structures
(B) RNA is a single stranded molecule
(C)RNA has uracil in place of thymidine
(D)RNA is susceptible to hydrolysis

2 Which one of the following modifications is common to both protein and DNA?
(A) SUMOylation

(B) Nitrosylation
(C) Methylation
(D) Ubiquitination

3 Protein A, which has strong affinity to Fc region of immunoglobulin, is extracted from (A)Saccharomyces cerevisiae
Staphylococcus aureus (C)Streptococcus pyogenes
(D)Streptococcus sanguis

4 The first humanized monoclonal antibody approved for the treatment of breast cancer is (A) Rituximab
(B) Cetuximab
(C) Bevacizumab
(D) Herceptin

5 Which one of the following aminoacids in proteins does NOT undergo phosphorylation?
(A) Ser
(B) Thr
(C) Pro
(D) Tyr

6 The role of an adjuvant is to (A) prolong the persistence of antigen
(B) cross link the antigen
(C) increase the size of antigen
(D) avoid inflammation

7 Endogenous antigens are presented on to the cell surface along with
(C) Fc γ receptor
(D) complement receptor

8 Human genome sequencing project involved the construction of genomic library in
(A)bacterial artificial chromosome
(C) bacteriophage
(D) pcDNA3.1

9 The nucleotide analogue used in DNA sequencing by chain termination method is
(A) 1',3'-dideoxy nucleoside triphosphate
(B) 2',3'-dideoxy nucleoside triphosphate
(C) 2',4'-dideoxy nucleoside triphosphate
(D) 2',5'-dideoxy nucleoside triphosphate

10 In nature, the horizontal gene transfer across bacteria is mediated by
(A) gene cloning followed by transformation
(B)conjugation and transformation
(C) conjugation only
(D) transformation only
Learn more:
1. (D)RNA is susceptible to hydrolysis
2. (C)Methylation
3. (B)Staphylococcus aureus
4. (D) Herceptin
Herceptin/Herclon (Trastuzumab) is a monoclonal antibody that interferes with the HER2/neu receptor. Its main use is to treat certain breast cancers.
5. (C) Pro
6. (A) prolong the persistence of antigen
7. (B) MHC-I
9.  (B) 2',3'-dideoxy nucleoside triphosphate

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