11. Statins are very effective against hypercholesterolemia, a major cause of atherosclerosis. These drugs reduce plasma cholesterol levels by
(A) Preventing absorption of cholesterol from the intestine.
(B) Increasing the excretion of cholesterol from the body via conversion to bile acids.
(C) Inhibiting the conversion of 3-hydroxy-3-methylglutaryl-CoA to mevalonate in the pathway for cholesterol biosynthesis.
(D) Increasing the rate of degradation of 3-hydroxy-3-methylglutaryl CoA reductase.
12. Measles, Mumps, Rubella-MMR combined vaccine represents which one of following vaccine categories?
(B) Live, attenuated
(D) Toxoid (inactivated toxin)
13. A haemophiliac man marries a normal woman. They have a daughter who does not show symptoms of haemophilia. If she marries a haemophiliac man, what will be the probability of their son displaying symptoms of haemophilia?
14. The conventional treatment for methanol toxicity is to administer ethanol. Which of the following explains the basis of this treatment?
(A) Ethanol acts as a competitive inhibitor to methanol
(B) Ethanol acts as a non-competitive inhibitor to methanol
(C) Ethanol destroys the enzymatic activity of alcohol dehydrogenase
(D) Ethanol blocks the entry of methanol within the cells.
15. Electrophoresis of a purified protein in SDS-PAGE in the presence of 2- mercaptoethanol yields two bands of 35 kDa and 45 kDa. However, in a gel filtration chromatography, the same protein elutes as 80 kDa. What conclusion will you draw from the results?
(A) The protein is not purified to homogeneity.
(B) Two bands generated in SDS-PAGE due to degradation.
(C) The protein is a homodimer
(D) The protein is a heterodimer
16. Lysosomes of a cell were labelled with lysotracker Red. Subsequently, these cells were infected with GFP-transfected Mycobacterium and observed under a fluorescence microscope. What will you observe?
(A) GFP-Mycobacterium will be colocalized with lysotracker Red labeled lysosomes.
(B) GFP-Mycobacterium will be separated from lysotracker Red labeled lysosomes.
(C) GFP-Mycobacterium will not be detected as they are degraded in the cell.
(D) Lysotracker Red labeled lysosomes will be degraded in GFP- Mycobacterium infected cells
17. A linear DNA fragment which has 3 restriction sites for BamH1, is labeled
/only at the 5 end. This DNA is partially digested with BamH1 in such a way that all kinds of fragments are generated. Under these conditions, how many labeled and unlabeled fragments will be produced?
(A) 3 labeled and 4 unlabeled
(B) 3 labeled and 5 unlabeled
(C) 4 labeled and 5 unlabeled
(D) 4 labeled and 6 unlabeled
18. In the preparation of humanized antibody, part of the antibody molecule is taken from mouse and the remaining is taken from that of human, through genetic engineering technique. Which one of the following statements is true for humanized antibody?
(A) CDRs of mouse IgG is fused with framework regions of human IgG
(B) CDRs of human IgG is fused with framework regions of mouse IgG
(C) CDRs of mouse IgG is fused with CDRs of human IgG
(D) framework regions of mouse IgG is fused with framework regions of
19. A 25-year old man undertakes a prolonged fast for religious reasons. Which one of the following metabolites will be elevated in his blood plasma after 24 hours?
(A) Lactic acid
(C) Ketone bodies
(D) Non-esterified fatty acids
20. Which one of the following is not a deficiency disorder?
(B) Night Blindness
(D) Pernicious Anemia
11. (C) Inhibiting the conversion of 3-hydroxy-3-methylglutaryl-CoA to mevalonate in the pathway for cholesterol biosynthesis.
12. (B) Live, attenuated
13. (C) 50%
14. (A) Ethanol acts as a competitive inhibitor to methanol
15. (D) The protein is a heterodimer
16. (A) GFP-Mycobacterium will be colocalized with lysotracker Red labeled lysosomes.
17. (D) 4 labeled and 6 unlabeled
18. (A) CDRs of mouse IgG is fused with framework regions of human IgG
19. (D) Non-esterified fatty acids
20. (C) Poliomyelitis