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DBT BET JRF Questions And Answers 2016

1. Which one of the following modes of inheritance is seen in Cystic Fibrosis
      
          (A)  Autosomal recessive  
          (B)  Autosomal dominant 
          (C)  Sex linked 
          (D)  Spontaneous mutation 

2.  Digestion of a 5Kb linear DNA fragment with EcoRI generates two fragments 
of 2 Kb and 3 Kb, while  digestion of the same molecule with HindIII yields three 
fragments of 0.7 Kb, 3.5 Kb and 0.8 Kb. When the same DNA is digested with 
both the enzymes, it yields fragments of 0.7 Kb, 1.3 Kb, 2.2 Kb and 0.8 Kb. The 
right sequence of restriction sites in the DNA fragment is  
      
       (A)  One EcoRI site in between two HindIII sites 
       (B)  One HindIII site in between two EcoRI sites 
       (C)  Two HindIII sites followed by only one EcoRI site 
       (D)  One EcoRI site followed by two HindIII sites. 
3.  GFP,  when  overexpressed  in  a  cell,  remains mostly  in  the  cytosol.  A  GFP 
construct is modified such that the resultant GFP protein will have a conjugated 
peptide Pro-Lys-Lys-Lys-Arg-Lys-Val at its N-terminus. If such a GFP construct is 
expressed in a cell, the modified GFP protein will be localized in the  
 
         (A)  lysosome 
         (B)  Golgi bodies 
         (C)  nucleus 
         (D)  endoplasmic reticulum    

4. In a mammalian cell, protein synthesis is regulated at the level of initiation by 
various  kinases.  During  viral  infection,  which  one  of  the  following  kinases  is 
involved  in  regulating  the  step  of  formation  of  eIF2.GTP.Met  tRNA   ternary 
icomplex in the host? 
      
          (A)  Heme-regulated inhibitor kinase (HRI) 
          (B)  Protein kinase RNA dependent (PKR) 
          (C)  GCN2-like kinase 
          (D)  PKR-like endoplasmic reticulum kinase (PERK) 
 
5. Which  one  of  the following  side  chains  of  an amino acid  is  responsible for 
fluorescence in proteins? 
          (A)  Indole ring 
          (B)  Guanidino group 
          (C)  Phenolic group 
          (D)  Imidazole group 
 
6. DNA molecules labeled with 15N and 14N can be separated by 
           (A) Pulse field gel electrophoresis 
           (B) Density gradient ultracentrifugation 
           (C) Capillary electrophoresis 
           (D) Differential centrifugation 
 
7. Cytoskeletal organization of a cell is regulated by 
      
          (A)  Ras GTPase 
          (B)  Rab GTPase 
          (C)  Rho GTPase 
          (D)  Ran GTPase 

8.  In  comparison to animals residing in a warm climate, animals living in cold 
climate need thermal insulation.  The  cell  membranes of the latter would have a 
relatively higher content of …… 
            (A)  sphinogolipid 
            (B)  saturated fatty acid 
            (C)  unsaturated fatty acid 
            (D)  cholesterol 
 
9. In glycolysis, the conversion of 1 mol of fructose 1,6-biphosphate to 2 mol of  
pyruvate results in the formation of 
 
            (A) 1 mol NAD+ and 2 mol of ATP 
            (B) 2 mol of NAD+ and 4 mol of ATP 
            (C) 2 mol of NADH and 2 mol of ATP 
            (D) 2 mol of NADH and 4 mol of ATP 

10. In diabetic ketoacidosis, increase in which of the following would cause 
elevated production of ketone bodies? 
      
          (A)  Proteolysis 
          (B)  Urea production 
          (C)  Insulin release 
          (D)  Lipolysis
Answers:
1. (A)  Autosomal recessive  
2. (A)  One EcoRI site in between two HindIII sites  
3. (C)  nucleus  
4. (B)  Protein kinase RNA dependent (PKR) 
5. (A)  Indole ring  
6. (B) Density gradient ultracentrifugation  
7. (C)  Rho GTPase  
8. (C)  unsaturated fatty acid  
9. (D) 2 mol of NADH and 4 mol of ATP  
10. (D)  Lipolysis

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