Cloning Plasmids - Used to facilitate the cloning of DNA fragments. Cloning vectors tend to be very simple, often containing only a bacterial resistance gene, origin and Multiple cloning sites.
Expression Plasmids - Used for gene expression. Expression vectors must contain a promoter sequence as well as a transcription terminator sequence, and the inserted gene. An expression vector can also include an enhancer sequence which increases the amount of protein or RNA produced.
Gene knock-down Plasmids - Used for reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA (small hairpin RNA) targeting the mRNA of the gene of interest.
![Types of plasmids Types of plasmids](//lh5.ggpht.com/-wfHrnmqNWJE/UswvwxhNDbI/AAAAAAAACho/Iitqu-ijwR4/clip_image002%25255B18%25255D.gif?imgmax=800)
Reporter Plasmids - Used for studying the function of genetic elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic element. For instance, a promoter of interest could be inserted upstream of the luciferase gene to determine the level of transcription driven by that promoter.
Viral Plasmids - These plasmids use technology evolved by viruses to aid in delivery of genetic material into target cells . You can use these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect target cells at a high efficiency.
Expression Plasmids - Used for gene expression. Expression vectors must contain a promoter sequence as well as a transcription terminator sequence, and the inserted gene. An expression vector can also include an enhancer sequence which increases the amount of protein or RNA produced.
Gene knock-down Plasmids - Used for reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA (small hairpin RNA) targeting the mRNA of the gene of interest.
![Types of plasmids Types of plasmids](http://lh5.ggpht.com/-wfHrnmqNWJE/UswvwxhNDbI/AAAAAAAACho/Iitqu-ijwR4/clip_image002%25255B18%25255D.gif?imgmax=800)
Reporter Plasmids - Used for studying the function of genetic elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic element. For instance, a promoter of interest could be inserted upstream of the luciferase gene to determine the level of transcription driven by that promoter.
Viral Plasmids - These plasmids use technology evolved by viruses to aid in delivery of genetic material into target cells . You can use these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect target cells at a high efficiency.