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Function of SDS in DNA extraction and in SDS PAGE

Sodium lauryl sulfate or Sodium dodecyl sulphate (SDS). SDS is a strong anionic detergent used to denature proteins.
  • Used in SDS-PAGE and  in DNA extraction procedure.
  • SDS-PAGE (Sodium dodecyl sulphate –polyacrylamide gel electrophoresis) is a technique for separating proteins based on size.
Mechanism of action:
function of SDS
  • In DNA extraction procedure, SDS is used for cell lysis and release of cell contents
  • In SDS PAGE, SDS has 2 function
         1. It denatures the protein
         2. It provides an overall negative change to the protein so that separation is based on size of the protein in SDS-PAGE.
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