Definition: In Feed-forward activation, a metabolite produced early in a pathway activates an enzyme that catalyze a reaction further down the pathwayFeed Forward activation in Glycolysis
Pyruvate kinase (Step 10 enzyme) is activated by Fructose-1,6-bisphosphate (3 rd step metabolite)
Fructose-1,6- bisphosphate is formed by the phosphorylation of fructose-6- bisphosphate by the enzyme Phosphofructokinase (PFK). This is the committed step or rate limiting step of Glycolysis. Excess Fructose-1,6- bisphosphate in the rate limiting step activates the formation of final product of the reaction; pyruvate by activating the enzyme in the final step; pyruvate kinase.
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