High-Pressure Liquid Chromatography (HPLC)
Using this chromatography technique it is possible to perform structural, and functional analysis, and purification of many molecules within a short time, This technique yields perfect results in the separation, and identification of amino acids, carbohydrates, lipids, nucleic acids, proteins, steroids, and other biologically active molecules.
In HPLC, mobile phase passes through columns under 10–400 atmospheric pressure, and with a high (0.1–5 cm//sec) flow rate. In this technique, use of small particles, and application of high pressure on the rate of solvent flow increases separation power, of HPLC and the analysis is completed within a short time.
