Recombinant DNA Methods Practice test | Methods in Biology

1.Chromosomes of Yeast and Protozoa are best separated using ?
Visible microscopy
Electon microscopy
PAGE
Pulse Field Gel Electrophoresis

2.DNA Fragment between size 2000 KD and 500 Kd can be separated using
PAGE
Chromatography
centrifugation
Pulse Field Gel Electrophoresis

3. YAC behaves similar to normal yeast chromosomes because it posses
Centromere
Centromere and Telomere
Telomere and ARS
Telomere,Centromere and ARS

4.Bacterial genomes is prevented by its own endonuclease by
immune mechanism
nuclease resistant genome
are not much effective on bacterial genom
methylation at restriction sites

5.Which one of the following statements regarding restriction/modifying enzymes used in recombinant. DNA technology is correct?
Endonucleases remove nucleotides, one at a time, from the ends of a sequence
Type ii class of restriction enzymes do not recohnise palindromic sequences.
Mung bean nuclease acts on double stranded DNA or RNA termini
Type ii class of restriction enzymes can generate either sticky or blunt ends

6.The virus inserted in genome can be recognised by
FISH
Northern Blot
Microarray
Southern Blot

7. Which of the following is not a co-dominant marker
RAPD
RFLP
SNP
VNTR

8.Which one of the following techniques/ approarches cannot be used to analyse expression pattern of a gene?
Western blotting
Southern Blotting
qRT PCR
Northern blotting

9.Which one of the following techniques will you use to identify more than 1000 differentially expressed genes in and tumor tissues in one experiment?
RAPD
Genome Sequencing
Chip assay
Transcriptome analysis

10. The presence and distribution of specific mRNAS within a cell be detected by
Northern blot analysis
RNASE Protection assay
in-situ hybridisation
real time PCR

Score =
Correct answers:

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