Biotechnology and Bioinformatics Notes and Videos

• What is Biotechnology

• Old Biotechnology vs New (Modern Biotechnology)

• Steps in rDNA technology or Recombinant DNA technology

• Gene Cloning Vector

• Cosmid and Lambda Vectors

• Shuttle Vector and Expression Vectors

• Cloning Vector and Expression Vectors

• Plasmids as Cloning Vector

• Types of Plasmid Vectors

• Genomic Library: Definition and Steps in the construction of Genomic Library

• Type I and Type II Restriction Enzymes

• Genomic Library vs cDNA library

• Gene cloning vs PCR

• Agrobacterium, Natural Genetic Engineer

• Organization of Ti plasmid

• Vir region or Virulence region of Ti plasmid

• Steps involved in Agrobacterium mediated gene transfer

• Principle behind Agrobacterium mediated gene transfer

• Ti plasmid Based Vectors- Cointegrate and Binary vectors

• Agrobacterium rhizhogenes and Ri plasmid

• What is Gene Therapy? Is it a treatment method for curing genetic disease.

• Somatic Gene Therapy and Germline Gene Therapy

• Limitations of Gene Therapy

• Difference between Somatic and Germline Gene Therapy

• Gene Replacement (Gene Augmentation) Therapy and Corrective Gene Therapy

• Approved Biotechnology Drugs (Mechanism of action)

• Mechanism of action of Insulin
• Enzymes in rDNA technology

Molecular Biology Techniques

• PCR:Requirements, Steps involved and Applications

• RAPD and RFLP

• Minisatellites and Microsatellites

• DNA Footprinting - Definition, Principle and Procedure

• Western Blotting: Principle, Procedure and Applications

• Northern Blotting: Principle, Procedure and Applications 

• Dot Blot Technique: Definition, Principle, Procedure and Applications

• Southern Blotting Principle, Procedure and Application

• Southern Blotting Procedure Steps

• Protein Fingerprinting Principle and Procedure

• DNA fingerprinting Procedure Application

• DNA Microarray Definition and Principle

• DNA Microarray Procedure and Applications

Bioinformatics Notes

• Bioinformatics Definition

• Why we need Bioinformatics

• Databases and Different types of Biological Databases

• Nucleotide Sequence Databases

• List of Model Organism Databases

For more:  Biotech Videos/Biotech MCQs/ Biotechnology Quiz

Last updated on 20-05-2014